7KTL

DNA Polymerase Mu (K438D), 8-oxodGTP:Ct Product State Ternary Complex, 50 mM Mn2+ (90min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.271, 62.099, 118.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.961 - 1.421
R-factor	0.1592
Rwork	0.158
R-free	0.17380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.440
High resolution limit [Å]	1.420	3.850	1.420
Rmerge	0.069	0.058	0.884
Rmeas	0.075	0.064	0.960
Rpim	0.030	0.027	0.371
Total number of observations	533551
Number of reflections	84301	4412	4203
<I/σ(I)>	12
Completeness [%]	99.5	96.9	100
Redundancy	6.3	5.6	6.6
CC(1/2)		0.993	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000