7KTF

DNA Polymerase Mu, 8-oxodGTP:Ct Product State Ternary Complex, 50 mM Mg2+ (180min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.947, 68.515, 110.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.970 - 1.489
R-factor	0.1684
Rwork	0.167
R-free	0.18710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.520
High resolution limit [Å]	1.489	4.040	1.490
Rmerge	0.081	0.055	0.959
Rmeas	0.090	0.061	1.075
Rpim	0.037	0.025	0.478
Number of reflections	73340	3960	3569
<I/σ(I)>	10.4
Completeness [%]	97.4	97.9	95.9
Redundancy	5.9	5.8	4.8
CC(1/2)		0.994	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000