7KTC

DNA Polymerase Mu, 8-oxodGTP:Ct Product State Ternary Complex, 10 mM Mn2+ (120min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-22
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.272, 62.354, 118.901
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.020 - 1.653
R-factor	0.1567
Rwork	0.156
R-free	0.17610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.650	4.480	1.650
Rmerge	0.098	0.082	0.709
Rmeas	0.108	0.091	0.806
Rpim	0.043	0.037	0.378
Total number of observations	319975
Number of reflections	54399	2933	2660
<I/σ(I)>	8.8
Completeness [%]	99.9	99.1	99.8
Redundancy	5.9	5.8	4.4
CC(1/2)		0.982	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000