7KTA

DNA Polymerase Mu, 8-oxodGTP:Ct Pre-Catalytic Ground State Ternary Complex, 20 mM Ca2+ (120min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.905, 68.718, 110.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.441 - 1.844
R-factor	0.1687
Rwork	0.167
R-free	0.19840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.870
High resolution limit [Å]	1.840	4.990	1.840
Rmerge	0.083	0.080	0.935
Rmeas	0.088	0.088
Rpim	0.034	0.035	0.398
Total number of observations	287020
Number of reflections	40039	2173	1984
<I/σ(I)>	10.4
Completeness [%]	100.0	99.3	99.9
Redundancy	7.2	6.6	6.3
CC(1/2)		0.992	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000