7KT7

DNA Polymerase Mu, 8-oxodGTP:At Reaction State Ternary Complex, 50 mM Mg2+ (60min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.908, 68.531, 110.589
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.951 - 1.760
R-factor	0.1627
Rwork	0.161
R-free	0.18650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.790
High resolution limit [Å]	1.760	4.780	1.760
Rmerge	0.110	0.055	0.935
Rmeas	0.123	0.062	1.055
Rpim	0.054	0.026	0.480
Total number of observations	233405
Number of reflections	45715	2489	2241
<I/σ(I)>	7.7
Completeness [%]	99.7	99.8	99.9
Redundancy	5.1	5.4	4.7
CC(1/2)		0.994	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000