7KT1

DNA Polymerase Mu, dGTP:At Reaction State Ternary Complex, 50 mM Mn2+ (180min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.754, 68.793, 110.223
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.011 - 1.665
R-factor	0.1658
Rwork	0.165
R-free	0.18860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.014
RMSD bond angle	1.293
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.700
High resolution limit [Å]	1.665	4.530	1.670
Rmerge	0.093	0.092	0.895
Rmeas	0.100	0.099	0.987
Rpim	0.038	0.037	0.408
Number of reflections	53801	2922	2600
<I/σ(I)>	11.6
Completeness [%]	99.8	99.9	99.3
Redundancy	7.1	7.1	5.7
CC(1/2)		0.981	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG 4000