7KSZ

DNA Polymerase Mu, dGTP:At Pre-Catalytic Ground State Ternary Complex, 10 mM Ca2+ (960min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.753, 68.778, 110.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.826 - 1.417
R-factor	0.1582
Rwork	0.157
R-free	0.17470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.010
RMSD bond angle	1.197
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.440
High resolution limit [Å]	1.417	3.850	1.420
Rmerge	0.097	0.081	0.919
Rmeas	0.108	0.091	1.021
Rpim	0.046	0.040	0.440
Number of reflections	86531	4587	4279
<I/σ(I)>	10.6
Completeness [%]	99.8	98.8	100
Redundancy	5.5	5.1	5.1
CC(1/2)		0.986	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG 4000