7KSY

DNA Polymerase Mu, dGTP:Ct Product State Ternary Complex, 10 mM Mg2+ (960min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-15
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.316, 62.323, 119.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.037 - 1.577
R-factor	0.1565
Rwork	0.156
R-free	0.16950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.008
RMSD bond angle	1.067
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.610
High resolution limit [Å]	1.577	4.290	1.580
Rmerge	0.095	0.092	0.569
Rmeas	0.104	0.101	0.638
Rpim	0.040	0.040	0.283
Number of reflections	62385	3357	3095
<I/σ(I)>	11
Completeness [%]	99.9	99.4	100
Redundancy	6.4	6.2	4.7
CC(1/2)		0.989	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000