7KSX

DNA Polymerase Mu, dGTP:Ct Product State Ternary Complex, 10 mM Mg2+ (30min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.011, 68.695, 110.522
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.832 - 1.570
R-factor	0.1645
Rwork	0.163
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.014
RMSD bond angle	1.273
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.600
High resolution limit [Å]	1.570	4.260	1.570
Rmerge	0.046	0.027	0.844
Rmeas	0.051	0.031	0.941
Rpim	0.022	0.014	0.409
Total number of observations	338599
Number of reflections	64263	3452	3146
<I/σ(I)>	12.6
Completeness [%]	99.7	99.1	99.5
Redundancy	5.3	5	5
CC(1/2)		0.998	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000