7KSS

DNA Polymerase Mu, dGTP:Ct Pre-Catalytic Ground State Ternary Complex, 10 mM Ca2+ (20min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-17
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.023, 68.767, 110.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.839 - 1.503
R-factor	0.1607
Rwork	0.160
R-free	0.17690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.189	0.213	0.937
Rmeas	0.203	0.229	1.045
Rpim	0.073	0.083	0.456
Total number of observations	497661
Number of reflections	71579	3724	3542
<I/σ(I)>	9
Completeness [%]	97.8	94.6	97.4
Redundancy	7	6.8	5.1
CC(1/2)		0.955	0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000