7KRW
Stimulating state of near full-length Hsp70 DnaK fused with a substrate peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-21 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | I 2 3 |
Unit cell lengths | 294.203, 294.203, 294.203 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.630 - 7.700 |
R-factor | 0.4549 |
Rwork | 0.304 |
R-free | 0.31640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7kru |
RMSD bond length | 0.009 |
RMSD bond angle | 1.371 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.630 | 39.380 | 7.980 |
High resolution limit [Å] | 7.700 | 15.660 | 7.700 |
Rmerge | 0.632 | 0.116 | 3.700 |
Rmeas | 0.644 | 0.123 | 3.770 |
Rpim | 0.121 | 0.041 | 0.740 |
Number of reflections | 5107 | 594 | 510 |
<I/σ(I)> | 8.97 | 0.56 | |
Completeness [%] | 97.1 | 92.5 | 99.2 |
Redundancy | 26.7 | 7.8 | 25.5 |
CC(1/2) | 0.947 | 0.989 | 0.410 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M HEPES pH 7, 3.2 M ammonium sulfate |