7KRI

FR6-bound SARS-CoV-2 Nsp9 RNA-replicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95374
Spacegroup name	P 32 2 1
Unit cell lengths	109.459, 109.459, 77.046
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.620 - 1.580
R-factor	0.1786
Rwork	0.178
R-free	0.19530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6w9q
RMSD bond length	0.006
RMSD bond angle	0.861
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.620	1.610
High resolution limit [Å]	1.580	1.580
Rpim	0.026	0.619
Number of reflections	73088	3604
<I/σ(I)>	13.4
Completeness [%]	100.0	99.9
Redundancy	5.2	10.5
CC(1/2)	0.999	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4	293	0.1M Sodium Citrate pH 4.0 2.2-2.4M Sodium Malonate