7KR6
Glycoside hydrolase family 16 endo-glucanase from Bacteroides ovatus in complex with G4G3G-2F-DNP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.447, 84.576, 67.105 |
| Unit cell angles | 90.00, 93.98, 90.00 |
Refinement procedure
| Resolution | 39.400 - 1.560 |
| Rwork | 0.172 |
| R-free | 0.19850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nbo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.707 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 143215 | 6822 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 98.7 | |
| Redundancy | 2 | |
| CC(1/2) | 1.000 | 0.935 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.2 M sodium chloride, 0.1 M Tris pH 8.5, 25% (w/v) PEG3350 |
