7KR3
Human DNA Ligase 1(E346A/E592A) Bound to a bulged DNA substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.681, 101.665, 115.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.840 - 2.778 |
R-factor | 0.1974 |
Rwork | 0.193 |
R-free | 0.23940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6p09 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.508 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.778 | 2.778 |
Number of reflections | 21959 | 1097 |
<I/σ(I)> | 21.53 | |
Completeness [%] | 97.5 | |
Redundancy | 6 | |
CC(1/2) | 0.924 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES, 150 mM lithium acetate, 10% (w/v) polyethylene glycol 3350 |