7KQX

MIF Y99C homotrimeric mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	93
Detector technology	PIXEL
Collection date	2015-04-29
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5428
Spacegroup name	P 21 21 21
Unit cell lengths	67.984, 68.062, 86.737
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.100 - 1.600
R-factor	0.1541
Rwork	0.153
R-free	0.17980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bs9
RMSD bond length	0.016
RMSD bond angle	1.344
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX ((1.18_3845: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.046	0.035	0.331
Rmeas	0.050	0.037	0.391
Rpim	0.018	0.011	0.204
Total number of observations	303731
Number of reflections	53669	2915	2564
<I/σ(I)>	16.3
Completeness [%]	99.6	100	97.9
Redundancy	5.7	10.6	3.4
CC(1/2)		0.999	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5