7KQD

Prefusion RSV F Bound to RV521

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 41 3 2
Unit cell lengths	168.610, 168.610, 168.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.742 - 2.940
R-factor	0.2077
Rwork	0.205
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ea4
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.060	45.060	3.120
High resolution limit [Å]	2.940	8.820	2.940
Rmerge	0.362	0.053	2.710
Rmeas	0.367	0.054	2.758
Rpim	0.063	0.010	0.506
Total number of observations	616976	23537	83346
Number of reflections	18058	785	2854
<I/σ(I)>	13.3	44.2	1.7
Completeness [%]	100.0	99.4	100
Redundancy	34.2	30	29.2
CC(1/2)	0.997	0.999	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M CHES pH 9.0, 0.2 M lithium sulfate and 1.77 M potassium/sodium tartrate