7KNJ
C1B domain of Protein kinase C in complex with Phorbol ester and Phosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2020-09-05 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 34.205, 26.138, 58.158 |
| Unit cell angles | 90.00, 96.92, 90.00 |
Refinement procedure
| Resolution | 19.240 - 1.570 |
| R-factor | 0.1799 |
| Rwork | 0.177 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ptq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.538 |
| Data reduction software | SAINT |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.700 | 1.670 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.055 | 0.205 |
| Number of reflections | 6195 | 414 |
| <I/σ(I)> | 21.05 | |
| Completeness [%] | 85.0 | 34 |
| Redundancy | 5.7 | 0.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | Screen condition: 0.2 M Ammonium acetate, 0.1 M Sodium phosphate, 30% Isopropanol, pH 6.8, Drop condition: Protein: 2 mM in MES pH 6.5, 150 mM KCl, Phosphatidylcholine: 20 mM, Phorbol ester: 2.5 mM |
