7KLJ

Crystal structure of the WD-repeat domain of human KIF21A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2020-09-20
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	55.275, 67.067, 79.880
Unit cell angles	90.00, 94.43, 90.00

Refinement procedure

Resolution	20.200 - 1.520
R-factor	0.1705
Rwork	0.170
R-free	0.18490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.439
Data scaling software	HKL-3000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.550
High resolution limit [Å]	1.520	4.130	1.520
Rmerge	0.073	0.044	0.424
Rmeas	0.078	0.048	0.487
Rpim	0.029	0.018	0.233
Total number of observations	600649
Number of reflections	89050	4569	3875
<I/σ(I)>	12.2
Completeness [%]	99.3	98.9	87.1
Redundancy	6.7	7	3.9
CC(1/2)		0.999	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	25% PEG3350, 0.2 M ammonium acetate, 0.1 M HEPES, pH 7.4