7KLF

Ternary structure of Dpo4 bound to G in the template base paired with incoming dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 21 21 2
Unit cell lengths	99.520, 102.373, 52.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.538 - 2.301
R-factor	0.2335
Rwork	0.231
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qz7
RMSD bond length	0.003
RMSD bond angle	0.628
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	10.000	2.340
High resolution limit [Å]	2.300	6.180	2.300
Rmerge	0.091	0.035	0.725
Total number of observations	263794
Number of reflections	24689	1360	1201
<I/σ(I)>	8.1
Completeness [%]	100.0	99.9	99.9
Redundancy	10.7	10.1	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	12 to 18% PEG 3350, 100 mM HEPES (pH 6.6),100 mM calcicum acetate, and 2.5% glycerol