7KL8

Structure of F420 binding protein Rv1558 from Mycobacterium tuberculosis with F420 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953719973564
Spacegroup name	P 43 3 2
Unit cell lengths	169.544, 169.544, 169.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.990 - 2.469
R-factor	0.20106
Rwork	0.200
R-free	0.22012
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r5r
RMSD bond length	0.009
RMSD bond angle	1.248
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.990	2.558
High resolution limit [Å]	2.469	2.469
Rmerge	0.187	5.963
Rpim	0.028	0.945
Number of reflections	30473	2899
<I/σ(I)>	28.5	1.1
Completeness [%]	99.7	97.54
Redundancy	78.5
CC(1/2)	1.000	0.416

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.1 M Sodium citrate pH 5.8, 0.5 M Ammonium sulfate, 1 M Lithium sulfate, and 3 % Glycerol (v/v)