7KKV

Crystal structure of Bacillus halodurans OapB in complex with its OLE RNA target (native, crystal form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-27
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	H 3 2
Unit cell lengths	98.947, 98.947, 197.852
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.875 - 2.000
R-factor	0.1703
Rwork	0.169
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7k9d
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.16_3549)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.840	42.840	2.000
High resolution limit [Å]	1.950	8.930	1.950
Rmerge	0.029	0.020	1.710
Rmeas	0.033	0.023	1.922
Rpim	0.015	0.012	0.867
Total number of observations	123752	1284	8739
Number of reflections	27246	315	1855
<I/σ(I)>	20.5	65.7	1
Completeness [%]	99.1	95.7	96.9
Redundancy	4.5	4.1	4.7
CC(1/2)	1.000	0.999	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	100 mM HEPES (pH 7.0 to 7.5), 200 mM NH4Cl and 39 to 42.5% 2-Methyl-2,4-pentanediol (MPD)