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7KJZ

crystal structure of PLEKHA7 PH domain biding inositol-tetraphosphate

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU R-AXIS
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2014-10-29
DetectorRIGAKU RAXIS HTC
Wavelength(s)1.5418
Spacegroup nameP 32 2 1
Unit cell lengths77.470, 77.470, 82.561
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution38.730 - 2.430
R-factor0.1848
Rwork0.182
R-free0.22310
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)7kjo
RMSD bond length0.013
RMSD bond angle1.891
Data reduction softwareiMOSFLM
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0257)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]52.1002.530
High resolution limit [Å]2.4302.430
Rmerge0.0580.560
Number of reflections111341136
<I/σ(I)>21.83.9
Completeness [%]99.9
Redundancy10.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP293protein in 2.5 mM I(1,3,4,5)P4, 180 mM NaCl, 20 mM Tris, 30 mM BisTris, 0.5 mM TCEP was mixed with 20% PEG 3350, 200 mM Na acetate

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