7KJZ
crystal structure of PLEKHA7 PH domain biding inositol-tetraphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU R-AXIS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-10-29 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 77.470, 77.470, 82.561 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.730 - 2.430 |
R-factor | 0.1848 |
Rwork | 0.182 |
R-free | 0.22310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7kjo |
RMSD bond length | 0.013 |
RMSD bond angle | 1.891 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.100 | 2.530 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.058 | 0.560 |
Number of reflections | 11134 | 1136 |
<I/σ(I)> | 21.8 | 3.9 |
Completeness [%] | 99.9 | |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | protein in 2.5 mM I(1,3,4,5)P4, 180 mM NaCl, 20 mM Tris, 30 mM BisTris, 0.5 mM TCEP was mixed with 20% PEG 3350, 200 mM Na acetate |