7KJZ

crystal structure of PLEKHA7 PH domain biding inositol-tetraphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-29
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	77.470, 77.470, 82.561
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.730 - 2.430
R-factor	0.1848
Rwork	0.182
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kjo
RMSD bond length	0.013
RMSD bond angle	1.891
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.100	2.530
High resolution limit [Å]	2.430	2.430
Rmerge	0.058	0.560
Number of reflections	11134	1136
<I/σ(I)>	21.8	3.9
Completeness [%]	99.9
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	protein in 2.5 mM I(1,3,4,5)P4, 180 mM NaCl, 20 mM Tris, 30 mM BisTris, 0.5 mM TCEP was mixed with 20% PEG 3350, 200 mM Na acetate