7KGK
Crystal structure of synthetic nanobody (Sb16) complexes with SARS-CoV-2 receptor binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 65.640, 65.640, 344.690 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.090 - 2.600 |
R-factor | 0.2589 |
Rwork | 0.258 |
R-free | 0.27640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bz5 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.506 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.340 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.080 | 0.455 |
Rpim | 0.024 | 0.134 |
Number of reflections | 13219 | 1185 |
<I/σ(I)> | 18 | 3.3 |
Completeness [%] | 98.8 | 99.1 |
Redundancy | 10.3 | 10.9 |
CC(1/2) | 0.999 | 0.987 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% PEG 20000, and 0.1M Hepes pH 7.0 |