7KG8

Structure of human PARG complexed with PARG-061

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	66.408, 89.201, 93.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.330 - 1.430
R-factor	0.1734
Rwork	0.172
R-free	0.19230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b1g
RMSD bond length	0.005
RMSD bond angle	0.866
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.330	1.481
High resolution limit [Å]	1.430	1.430
Rmerge	0.064	2.646
Rmeas	0.068	2.826
Rpim	0.024	0.980
Number of reflections	103197	10175
<I/σ(I)>	13.49	0.81
Completeness [%]	99.6	98.92
Redundancy	7.8	8.1
CC(1/2)	0.999	0.393

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 M PCTP, pH 7.5, 0.2 M AmSO4, 18-23% PEG3350