7KG1

Structure of human PARG complexed with PARG-002

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	66.463, 88.964, 95.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.660 - 1.650
R-factor	0.1753
Rwork	0.174
R-free	0.19810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b1g
RMSD bond length	0.014
RMSD bond angle	1.109
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.660	1.709
High resolution limit [Å]	1.650	1.650
Rmerge	0.056	1.655
Rmeas	0.058	1.719
Rpim	0.016	0.461
Number of reflections	68294	6747
<I/σ(I)>	24.76	1.74
Completeness [%]	99.7	99.69
Redundancy	13.1	13.5
CC(1/2)	1.000	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 M PCTP, pH 7.5, 0.2 M AmSO4, 18-23% PEG3350