7KG0

Structure of human PARG complexed with PARG-131

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	45.293, 66.579, 89.142
Unit cell angles	90.00, 95.50, 90.00

Refinement procedure

Resolution	37.330 - 1.660
R-factor	0.1863
Rwork	0.185
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b1g
RMSD bond length	0.007
RMSD bond angle	0.854
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.920	1.719
High resolution limit [Å]	1.660	1.660
Rmerge	0.098	1.141
Rmeas	0.112	1.306
Rpim	0.053	0.620
Number of reflections	59073	4954
<I/σ(I)>	7.85	1.55
Completeness [%]	94.3	79.57
Redundancy	4.3	4.2
CC(1/2)	0.994	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 M PCTP, pH 7.5, 0.2 M AmSO4, 18-23% PEG3350