7KEF

RNA polymerase II elongation complex with unnatural base dTPT3, rNaM in swing state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	167.713, 221.711, 193.255
Unit cell angles	90.00, 101.78, 90.00

Refinement procedure

Resolution	48.700 - 3.890
R-factor	0.2701
Rwork	0.268
R-free	0.32550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e2j
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.701	3.990
High resolution limit [Å]	3.890	3.890
Rmerge	1.008	5.216
Rmeas	1.085	5.607
Rpim	0.400	2.049
Total number of observations		33419
Number of reflections	63384	4484
<I/σ(I)>	2.5	0.4
Completeness [%]	99.9	99.9
Redundancy	7.4	7.5
CC(1/2)	0.904	0.296

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000