7KE1
Factor H enhancing human antibody fragment (Fab) to meningococcal Factor H binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.11583 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.771, 97.260, 70.472 |
| Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
| Resolution | 70.470 - 1.500 |
| R-factor | 0.1623 |
| Rwork | 0.162 |
| R-free | 0.18260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.265 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.470 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.167 | 1.012 |
| Rmeas | 0.180 | 1.086 |
| Rpim | 0.067 | 0.394 |
| Number of reflections | 143102 | 97061 |
| <I/σ(I)> | 12.18 | 1.89 |
| Completeness [%] | 98.1 | 96.35 |
| Redundancy | 6.9 | 7 |
| CC(1/2) | 0.998 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | PEG 3350, Bis-Tris Propane, lithium sulfate |
