7KDF

Structure of Stu2 Bound to dwarf Ndc80c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	.97918
Spacegroup name	C 2 2 21
Unit cell lengths	170.886, 183.175, 124.317
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.720 - 2.720
R-factor	0.1993
Rwork	0.198
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tcs
RMSD bond length	0.004
RMSD bond angle	0.734
Data reduction software	HKL-2000
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.060	2.817
High resolution limit [Å]	2.720	2.720
Rmerge	0.107	0.904
Rmeas	0.111	0.940
Rpim	0.030	0.030
Number of reflections	52106	62919
<I/σ(I)>	18.4
Completeness [%]	99.0	91.15
Redundancy	13.4	13.3
CC(1/2)	0.999	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	288.15	1.3 M Ammonium Sulfate 0.1 M Hepes pH 7.3