7KCZ

CRYSTAL STRUCTURE OF RHESUS MACAQUE (MACACA MULATTA) IGG1 FC FRAGMENT- FC-GAMMA RECEPTOR III COMPLEX V158 MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	215.690, 70.110, 135.410
Unit cell angles	90.00, 119.77, 90.00

Refinement procedure

Resolution	60.110 - 3.150
R-factor	0.271
Rwork	0.269
R-free	0.30320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mj3
RMSD bond length	0.010
RMSD bond angle	1.548
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.110	3.320
High resolution limit [Å]	3.150	3.150
Rpim	0.101	0.605
Number of reflections	92773	4297
<I/σ(I)>	3
Completeness [%]	96.4	96.9
Redundancy	3.2	3.1
CC(1/2)	0.991	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294	20% PEG 4000, 0.2 M lithium sulfate, 0.1 M MES pH 6.0