7KC8

Salivary protein from Culex quinquefasciatus that belongs to the Cysteins and Tryptophan-Rich (CWRC) family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	95
Detector technology	CCD
Collection date	2020-02-26
Detector	RIGAKU SATURN A200
Wavelength(s)	1.541800
Spacegroup name	P 21 21 21
Unit cell lengths	72.940, 73.050, 106.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.740 - 2.300
R-factor	0.2087
Rwork	0.205
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	homology
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.740	26.740	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.031	0.021	0.201
Rmeas	0.032	0.022	0.215
Number of reflections	25008	331	1367
<I/σ(I)>	59.38	103.53	8.92
Completeness [%]	96.1	97.1	71.8
Redundancy	11.458	9.417	7.963
CC(1/2)	1.000	1.000	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M Sodium acetate trihydrate, 0.1M TRIS hydrochloride pH 8.5, 30% w/v PEG 4000