7KBL

Biotin Carboxylase domain of Thermophilic 2-Oxoglutarate Carboxylase bound to Bicarbonate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	67.496, 125.154, 125.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.746 - 2.300
R-factor	0.1833
Rwork	0.181
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 1ULZ
RMSD bond length	0.013
RMSD bond angle	1.158
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.240	2.240
Number of reflections	51900	2693
<I/σ(I)>	20.8
Completeness [%]	99.8	98.8
Redundancy	6.9	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	PEG 3350, 0.02M Citric Acid, 0.08M Bis-Tris-Propane