7K9R
Cryptococcus neoformans Hsp90 nucleotide binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.230, 80.260, 149.890 |
| Unit cell angles | 90.00, 92.32, 90.00 |
Refinement procedure
| Resolution | 29.950 - 1.950 |
| R-factor | 0.1692 |
| Rwork | 0.168 |
| R-free | 0.20040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cji |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.179 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.950 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.030 | 0.333 |
| Number of reflections | 48718 | 4717 |
| <I/σ(I)> | 16.76 | 3.14 |
| Completeness [%] | 85.2 | 83.4 |
| Redundancy | 4.3 | 3.8 |
| CC(1/2) | 0.999 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1M LiCL, Hepes pH7, 20-25% PEG 6K, 10-20% DMSO |
