7K9E

Crystal structure of Bacillus halodurans OapB in complex with its OLE RNA target (crystal form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-31
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65
Unit cell lengths	71.990, 71.990, 223.905
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.345 - 2.100
R-factor	0.1734
Rwork	0.172
R-free	0.20400
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.370	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.048	0.926
Rmeas	0.057	1.087
Rpim	0.030	0.565
Number of reflections	37999	3797
<I/σ(I)>	17.2	1.2
Completeness [%]	99.6	99.3
Redundancy	3.5	3.6
CC(1/2)	0.998	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	240 mM sodium malonate (pH 6.0 to 7.0) and 20 to 21% PEG 3350