7K9D

Crystal structure of Bacillus halodurans OapB in complex with its OLE RNA target (crystal form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-31
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.6058
Spacegroup name	H 3 2
Unit cell lengths	98.817, 98.817, 198.897
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.832 - 2.098
R-factor	0.1636
Rwork	0.162
R-free	0.19830
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.840	2.180
High resolution limit [Å]	2.098	2.098
Rmerge	0.050	1.211
Rmeas	0.053	1.280
Rpim	0.017	0.411
Number of reflections	42266	2175
<I/σ(I)>	29.9	1.7
Completeness [%]	99.9	100
Redundancy	9.8	9.4
CC(1/2)	0.994	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	100 mM HEPES (pH 7.0 to 7.5), 200 mM NH4Cl and 39 to 42.5% 2-Methyl-2,4-pentanediol (MPD)