7K97
Human DNA polymerase beta dGDP product complex with Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-05 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.102, 77.309, 55.111 |
| Unit cell angles | 90.00, 114.85, 90.00 |
Refinement procedure
| Resolution | 30.580 - 2.400 |
| R-factor | 0.1907 |
| Rwork | 0.185 |
| R-free | 0.23970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.089 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.19rc5_4047) |
| Refinement software | PHENIX (1.19rc5_4047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.093 | 0.961 |
| Rpim | 0.043 | 0.517 |
| Number of reflections | 16511 | 804 |
| <I/σ(I)> | 15.3 | 1.2 |
| Completeness [%] | 99.5 | 97.3 |
| Redundancy | 4.2 | 2.8 |
| CC(1/2) | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 50 mM imidazole, 350 mM sodium acetate, 18% PEG3350 |
