7K95

Crystal structure of human CPSF30 in complex with hFip1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 64
Unit cell lengths	79.040, 79.040, 48.662
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.550 - 1.900
R-factor	0.1811
Rwork	0.179
R-free	0.20920
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.984
Data reduction software	XDS (20180808)
Data scaling software	Aimless (0.7.4)
Phasing software	SHELX
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.660	48.660	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.046	0.034	0.874
Rmeas	0.048	0.035	0.917
Rpim	0.013
Total number of observations	174010
Number of reflections	13793	137	869
<I/σ(I)>	30.9
Completeness [%]	100.0	98.6	99.9
Redundancy	12.6	11.6	10.9
CC(1/2)	1.000	0.999	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	293	0.1 M sodium malonate, 16% (w/v) PEG 3350