7K8P
Crystal structure of an anti-SARS-CoV-2 human neutralizing antibody Fab fragment, C110
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.498, 62.738, 78.423 |
| Unit cell angles | 90.00, 97.51, 90.00 |
Refinement procedure
| Resolution | 38.690 - 1.800 |
| R-factor | 0.1981 |
| Rwork | 0.196 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xca |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.087 | 1.070 |
| Rpim | 0.041 | 0.820 |
| Number of reflections | 42891 | 4284 |
| <I/σ(I)> | 7.7 | 2 |
| Completeness [%] | 98.8 | 78.2 |
| Redundancy | 4.7 | 2 |
| CC(1/2) | 0.992 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 298 | 0.2 M sodium tartrate dibasic dihydrate, 20 % w/v PEG3350 |
