7K86

Crystal Structure of Glutamyl-tRNA synthetase (gltX) from Stenotrophomonas maltophilia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2020-03-05
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	85.400, 69.110, 103.820
Unit cell angles	90.00, 110.06, 90.00

Refinement procedure

Resolution	36.260 - 2.050
R-factor	0.1833
Rwork	0.182
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h4v
RMSD bond length	0.006
RMSD bond angle	0.802
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.260	36.260	2.100
High resolution limit [Å]	2.050	9.170	2.050
Rmerge	0.039	0.025	0.434
Rmeas	0.045	0.030	0.523
Total number of observations	293786
Number of reflections	71304	826	5087
<I/σ(I)>	18.49	43.47	2.42
Completeness [%]	99.6	96.3	97.4
Redundancy	4.12	3.735	3.23
CC(1/2)	0.999	0.998	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	StmaA.01187.b.B1.PW38726 at 24 mg/ml mixed 1:1 with MCSG1(c5): 10%(w/v) PEG-3350, 0.2 M magensium acetate. Tray: 313794c5. Puck: xku5-10.