7K7M

Crystal Structure of a membrane protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	180.378, 147.710, 138.192
Unit cell angles	90.00, 120.89, 90.00

Refinement procedure

Resolution	98.660 - 3.330
R-factor	0.2696
Rwork	0.267
R-free	0.32100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6or2
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	PHENIX (1.8)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.460
High resolution limit [Å]	3.330	7.200	3.340
Rmerge	0.089	0.031	3.041
Rmeas	0.101	0.035	3.442
Rpim	0.046	0.016	1.578
Number of reflections	44875	4554	4427
<I/σ(I)>	5.2
Completeness [%]	99.1	98.3	99.3
Redundancy	4.6	4.5	4.6
CC(1/2)		0.998	0.170

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	20mM Tris, 100mM NaAC and 25% PEG400