7K5U
Bst DNA polymerase I time-resolved structure, 48 hr post dATP and dCTP addition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-06-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.299, 93.491, 105.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.060 - 2.000 |
R-factor | 0.1907 |
Rwork | 0.190 |
R-free | 0.22950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dsy |
RMSD bond length | 0.007 |
RMSD bond angle | 0.842 |
Data reduction software | autoXDS |
Data scaling software | autoXDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.060 | 2.071 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.064 | 0.164 |
Rmeas | 0.070 | 0.180 |
Rpim | 0.028 | 0.074 |
Number of reflections | 58064 | 5685 |
<I/σ(I)> | 20.31 | |
Completeness [%] | 98.2 | |
Redundancy | 6.3 | |
CC(1/2) | 0.997 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50% ammonium sulfate, 0.1M MES, 2% MPD |