7K5P

Bst DNA polymerase I time-resolved structure, 4 min post dATP addition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	87.540, 93.301, 105.494
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.350 - 1.970
R-factor	0.2201
Rwork	0.219
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dsy
RMSD bond length	0.008
RMSD bond angle	0.924
Data reduction software	autoXDS
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.350	2.040
High resolution limit [Å]	1.970	1.970
Rmerge	0.048	0.186
Rmeas	0.055	0.210
Rpim	0.026	0.096
Number of reflections	60952	6101
<I/σ(I)>	21.97
Completeness [%]	97.7
Redundancy	4.6
CC(1/2)	0.998	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% ammonium sulfate, 0.1M MES, 2% MPD