7K5D

Ebola virus VP40 octameric ring generated by a DNA oligonucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100.5
Detector technology	PIXEL
Collection date	2019-04-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 4 2 2
Unit cell lengths	80.438, 80.438, 47.281
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.760 - 1.780
R-factor	0.1827
Rwork	0.182
R-free	0.19810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ldm
RMSD bond length	0.007
RMSD bond angle	0.929
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.760	1.820
High resolution limit [Å]	1.780	1.780
Rmerge	0.098	1.411
Number of reflections	15385	844
<I/σ(I)>	15.5	2.4
Completeness [%]	99.9	98.8
Redundancy	12.3	11.8
CC(1/2)	0.998	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	1 uL 3.5 mg/mL protein, 1 uL 75 mM sodium citrate tribasic pH 6.6, 150 mM ammonium acetate