7K4I
Human Arginase 1 in complex with compound 06.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.700, 287.100, 67.150 |
| Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
| Resolution | 71.780 - 1.980 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6v7e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.780 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 125565 | 12199 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 89.3 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.994 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% MMT (pH 7.0) 0.1 M ammonium formate, 16-22% PEG 8000 |
