7K3L

Crystal structure of dLC8 in complex with Panoramix TQ peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-02
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	I 41 2 2
Unit cell lengths	62.900, 62.900, 120.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.750 - 1.790
R-factor	0.178
Rwork	0.176
R-free	0.20950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zke
RMSD bond length	0.007
RMSD bond angle	0.857
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.200	1.890
High resolution limit [Å]	1.790	1.790
Rmerge	0.093	0.415
Number of reflections	11305	1281
<I/σ(I)>	14.2
Completeness [%]	96.1
Redundancy	8.6
CC(1/2)	0.997	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M KSCN, 30% (w/v) PEG 2000 MME