7K1O
Crystal Structure of NSP15 Endoribonuclease from SARS CoV-2 in the Complex with Uridine-3',5'-Diphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-25 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.460, 151.321, 197.518 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.680 - 2.400 |
| R-factor | 0.2086 |
| Rwork | 0.207 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 6VWW |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.595 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.680 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.144 | 1.497 |
| Number of reflections | 49708 | 2066 |
| <I/σ(I)> | 15 | 0.76 |
| Completeness [%] | 96.8 | 81.1 |
| Redundancy | 7 | 4.1 |
| CC(1/2) | 0.993 | 0.463 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 8 % w/v Polyethylene glycol 4,000, 100 mM Sodium acetate; pH 4.6 |
