7K16

Tamana Bat Virus xrRNA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-02-25
Detector	RDI CMOS_8M
Wavelength(s)	1.0972
Spacegroup name	P 31 2 1
Unit cell lengths	79.100, 79.100, 40.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.550 - 2.100
R-factor	0.1918
Rwork	0.187
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oiu
RMSD bond length	0.009
RMSD bond angle	1.239
Data reduction software	XDS (VERSION Mar 15, 2019 BUILT=20191015)
Data scaling software	XDS (VERSION Mar 15, 2019 BUILT=20191015)
Phasing software	PHENIX (1.16_3549)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.550	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.095	1.694
Rmeas	0.097	1.739
Rpim	0.021	0.391
Number of reflections	8806	875
<I/σ(I)>	27.9	2.15
Completeness [%]	99.9	100
Redundancy	21.2	19.6
CC(1/2)	1.000	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Mg2+chloride hexahydrate, 0.1M HEPES sodium (pH 7.5), and 30% v/v Polyethylene glycol 400