7K07

Crystal structures and ribonuclease activity of the Flavivirus host factor ERI3 that is involved in viral RNA synthesis define the ERI subfamily of structure-specific 3-prime - 5-prime exoribonucleases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-22
Detector	PSI PILATUS 6M
Wavelength(s)	1.005
Spacegroup name	P 21 21 21
Unit cell lengths	58.627, 74.777, 96.725
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.390 - 2.150
R-factor	0.1791
Rwork	0.177
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xri
RMSD bond length	0.005
RMSD bond angle	0.742
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.400	2.190
High resolution limit [Å]	2.150	2.150
Rmeas	0.161
Rpim	0.051
Number of reflections	44601	2173
<I/σ(I)>	39.2
Completeness [%]	99.8
Redundancy	9
CC(1/2)		0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	35% MPEG 2K, 20% saturated KCl, 200 mM pH 8.0 Imidazole Malate, 5mM MnCl2, 20mM AMP