7JZS

Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	BRUKER METALJET
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-12
Detector	Bruker PHOTON II
Wavelength(s)	1.34
Spacegroup name	P 43 21 2
Unit cell lengths	58.720, 58.720, 120.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.750 - 1.300
R-factor	0.14039
Rwork	0.138
R-free	0.18425
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5us1
RMSD bond length	0.031
RMSD bond angle	2.528
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.750	1.346
High resolution limit [Å]	1.300	1.300
Number of reflections	52444	5117
<I/σ(I)>	28.09	2.55
Completeness [%]	100.0
Redundancy	12.1
CC(1/2)	1.000	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295.15	ammonium sulfate, PEG 400, HEPES