7JZJ
Crystal structure demonstrating CTD-CTD interactions of Zaire Ebola virus VP40 dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 85 |
| Detector technology | PIXEL |
| Collection date | 2010-05-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 62 |
| Unit cell lengths | 159.940, 159.940, 89.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.170 - 2.460 |
| R-factor | 0.2497 |
| Rwork | 0.249 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ldb |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.950 |
| Data reduction software | XDS (Jan 31, 2020) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.970 | 79.970 | 2.590 |
| High resolution limit [Å] | 2.460 | 7.780 | 2.460 |
| Rmerge | 0.098 | 0.021 | 3.368 |
| Rmeas | 0.105 | 0.023 | 3.598 |
| Rpim | 0.037 | 0.008 | 1.261 |
| Total number of observations | 350132 | 11710 | 55437 |
| Number of reflections | 44360 | 1584 | 6904 |
| <I/σ(I)> | 15.3 | 68.5 | 0.9 |
| Completeness [%] | 93.1 | 99.9 | 99.8 |
| Redundancy | 7.9 | 7.4 | 8 |
| CC(1/2) | 0.999 | 1.000 | 0.443 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.05 M magnesium chloride, 0.1 M HEPES, 38% PEG400, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
